Modulation of Postendocytic Sorting of G Protein-Coupled Receptors
Top Cited Papers
- 26 July 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 297 (5581) , 615-620
- https://doi.org/10.1126/science.1073308
Abstract
Recycling of the mu opioid receptor to the plasma membrane after endocytosis promotes rapid resensitization of signal transduction, whereas targeting of the delta opioid receptor (DOR) to lysosomes causes proteolytic down-regulation. We identified a protein that binds preferentially to the cytoplasmic tail of the DOR as a candidate heterotrimeric GTP-binding protein (G protein)–coupled receptor-associated sorting protein (GASP). Disruption of the DOR-GASP interaction through receptor mutation or overexpression of a dominant negative fragment of GASP inhibited receptor trafficking to lysosomes and promoted recycling. The GASP family of proteins may modulate lysosomal sorting and functional down-regulation of a variety of G protein–coupled receptors.Keywords
This publication has 25 references indexed in Scilit:
- Endocytosis of G protein-coupled receptors: roles of G protein-coupled receptor kinases and ß-arrestin proteinsProgress in Neurobiology, 2001
- A Transplantable Sorting Signal That Is Sufficient to Mediate Rapid Recycling of G Protein-coupled ReceptorsJournal of Biological Chemistry, 2001
- Binding of the β2 Adrenergic Receptor toN-Ethylmaleimide-sensitive Factor Regulates Receptor RecyclingPublished by Elsevier ,2001
- A Phosphorylation-regulated Brake Mechanism Controls the Initial Endocytosis of Opioid Receptors but Is Not Required for Post-endocytic Sorting to LysosomesPublished by Elsevier ,2001
- Newly Synthesized Human δ Opioid Receptors Retained in the Endoplasmic Reticulum Are Retrotranslocated to the Cytosol, Deglycosylated, Ubiquitinated, and Degraded by the ProteasomeJournal of Biological Chemistry, 2001
- Type-specific Sorting of G Protein-coupled Receptors after EndocytosisJournal of Biological Chemistry, 2000
- Immunisation contre Neisseria gonorrhoeae et Neisseria meningitidis (RI Arko et al, US)Biofutur, 1998
- Role of Clathrin-mediated Endocytosis in Agonist-induced Down-regulation of the β2-Adrenergic ReceptorJournal of Biological Chemistry, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- A Novel Interaction between Adrenergic Receptors and the α-Subunit of Eukaryotic Initiation Factor 2BPublished by Elsevier ,1997