Regulation of the Bacillus subtilis Extracytoplasmic Function Protein σ Y and Its Target Promoters
Open Access
- 15 August 2003
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (16) , 4883-4890
- https://doi.org/10.1128/jb.185.16.4883-4890.2003
Abstract
The Bacillus subtilis extracytoplasmic function sigma factor σ Y is of unknown function. We demonstrate that the sigY operon is expressed from an autoregulatory promoter site, P Y . We selected for transposon-induced mutations that upregulate P Y transcription in an attempt to identify genes involved in σ Y regulation. The resulting insertions disrupted yxlC , the gene immediately downstream of sigY . However, the phenotype of the yxlC ::Tn 10 insertion was due to polarity on the downstream genes of the sigY operon; a nonpolar insertion in yxlC did not lead to derepression of P Y . Further analyses revealed that both yxlD and yxlE encoded proteins important for the negative regulation of σ Y activity. A comparison of the transcriptomes of wild-type and yxlC ::Tn 10 mutant strains revealed elevated expression of several operons. However, only one additional gene, ybgB , was unambiguously identified as a direct target for σ Y . This was supported by analysis of direct targets for σ Y transcription with whole-genome runoff transcription followed by macroarray analysis.Keywords
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