Osmotic Control of Vasopressin Release by Rat Hypothalamo-Neurohypophyseal Explants in Organ Culture

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Abstract
The rat hypothalamo-neurohypophyseal system (HNS) in organ culture was used as an in vitro system for studying the osmotic control of vasopressin (VP) release. The HNS retains osmotically sensitive components as demonstrated by changes in the rate of VP release following alterations in the osmolality of the culture medium. Increasing the osmolality from 295 to 305 mosmol/kg H2O by the addition of NaCl resulted in a 2.5-fold increase in VP release. VP release was significantly decreased subsequent to reducing the osmolality from 295 to 280 mosmol/kg H2O by the addition of distilled water. VP release was stimulated when the osmolality was increased to 300 mosmol/kg H2O by the addition of mannitol, but not by additions of urea or glucose which resulted in comparable increases in the tonicity of the culture medium. The HNS in organ culture responds appropriately to osmotic challenges within the physiological range. Verney''s concept of an osmoreceptor is supported inasmuch as both NaCl and mannitol were effective osmotic agents.