Monoclonal Antibody-Based Detection System for Salmonella enteritidis
- 1 April 1993
- journal article
- research article
- Published by JSTOR
- Vol. 37 (2) , 501-7
- https://doi.org/10.2307/1591678
Abstract
Two monoclonal antibodies that react with Salmonella enteritidis in chicken tissue, eggs, and environmental samples were used to develop a rapid enzyme-linked immunosorbent assay (ELISA) screen and agglutination assay for the specific detection of S. enteritidis. S. enteritidis was detected in 100% of egg samples and 99.8% of various field and research samples by both ELISA and traditional microbiological isolation and identification techniques. Results of titer experiments indicate that as few as 10(4) organisms can be detected by ELISA.Keywords
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