THE GENETICS OF ARTEMIA SALINA. III. EFFECTS OF X-IRRADIATION AND OF FREEZING UPON CYSTS

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Abstract
Brine shrimp cysts were stored in a freezer or at room temperature. Viability of the frozen cysts remained unchanged whereas that of the unfrozen cysts declined. A 50,000 r dose of X-irradiation administered to the cysts did not decrease viability during the stages of gastnilation or embryonic differentiation but instead killed the metanauplius during later developmental stages (the second through the eighth instars). A dose of 10,000 r did not impair the viability of the cysts or the survival of hatched nauplii to adulthood. Although many aberrant characteristics were observed in the inbred offspring of irradiated shrimp, only one viable pure-breeding mutant shock was developed. It is characterized by garnet eye color which is determined by a recessive autosomal gene.