Biliary excretion of estradiol-17 beta-glucuronide in the rat

Publisher Website
Google Scholar
Abstract
Estradiol-17beta-glucuronide (E 2 17G) is a cholestatic agent and is considered to be related to the pathogenesis of intrahepatic cholestasis of pregnancy. In the current study, we examined the mechanism of the biliary excretion of E 2 17G and estradiol metabolites in rats. Biliary excretion of tracer doses of [ 3 H]estradiol-17beta-glucuronide and [ 14 C]estradiol or [ 3 H]taurocholate and ] 14 C]vinblastine, a P-glycoprotein (P-GP) substrate, intravenously administered as a bolus to bile-drained control rats or EHBR was studied. Biliary excretion of E 2 17G and estradiol metabolites EHBR was markedly delayed. Analyses of biliary metabolites after estradiol injection showed less polar conjugates in EHBR. In contrast, the excretion of taurocholate and vinblastine (VLB) was only slightly delayed in EHBR. Although phenothiazine treatment to induce the expression of P-GP increased biliary vinblastine excretion, it did not affect biliary excretion of a tracer dose of [ 3 H]estradiol-17beta-glucuronide. However, phenothiazine treatment inhibited the cholestasis induced by E 2 17G infused at the rate of 0.075 micromol/min/100g for 20 minutes and increased biliary E 2 17G excretion. Sulfobromophthalein infusion (0.2 micromol/min/100 g body 0 weight) markedly inhibited the biliary excretion of E 2 17G and estradiol metabolites, whereas dibromosulfophthalein (DBSP) at the same infusion rate had no effect. These findings indicate that EG17G is excreted into bile by a canalicular organic anion carrier for sulfobromopthalein (BSP), not for DBSP, under physiological conditions, and that P-GP influences E 2 17G excretion only at a high dose. under physiological conditions, and that P-GP influence s E 2 17G excretion only at a high dose. (Hepatology 1996 Mar;23(3):607-13)

This publication has 0 references indexed in Scilit: