Conformation of the hypervariable region L3 without the key proline residue
Open Access
- 1 January 1996
- journal article
- for the-record
- Published by Wiley in Protein Science
- Vol. 5 (1) , 167-169
- https://doi.org/10.1002/pro.5560050121
Abstract
The refined structure of the Fab fragment of the monoclonal antibody CRIS-I (IgG2ak) against the leukocyte differentiation antigen CD5, determined at 1.9 Å resolution with an agreement R-factor of 18.3%, reveals a variant of the canonical conformations proposed for the light chain complementarity determining region L3 (CDR-L3). This is the first Fab structure available with a K light chain in which the CDR-L3 lacks the key proline residue in either position 94 or 95. The conformation found could be significant for about 10% of the murine IgG molecules with k light chains without proline in their CDR-L3 sequences.Keywords
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