Ordered differential display: a simple method for systematic comparison of gene expression profiles
Open Access
- 1 June 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (12) , 2541-2542
- https://doi.org/10.1093/nar/25.12.2541
Abstract
A method for display of 3′-end restriction fragments of cDNAs is proposed, extending the idea reported recently. First, representative pools of such fragments are selectively amplified using PCR suppression effect. Then, simplified subsets of these fragments suitable for comparison by PAGE are amplified by adapter-specific primers extended by two randomly picked bases at their 3′ ends. By testing all possible combinations of extended primers the whole mRNA pool may be systematically investigated. The method was applied to search for molecular regional markers of freshwater planarian Dugesia tigrina.Keywords
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