Use of the Spectrophotometer for Measuring Melanin Dispersion in the Frog

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Abstract
The spectrophotometer was used as an instrument for recording the effects of mel-anophore expansion on the reflecting power of the skin pigments of the frog. The reflectance in the light-adapted frogs may be as much as 10% above that of the dark-adapted frogs at the same wave length. Melanophore expansion in dark- adapted frogs masks the reflecting power of the skin pigment at the red end of the spectrum more than at the violet. The avg. skin reflectance in the light-adapted frogs is not more than 21.8%. A difference between the response of melano-phores to environmental conditions and operations of the hypophysis is suggested.

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