ZONATION OF THE ADRENAL CORTEX II. EFFECT OF BSA ON COUPLING EFFICIENCY OF MITOCHONDRIA ISOLATED FROM THE ZONA GLOMERULOSA OF THE BOVINE ADRENAL CORTEX

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Abstract
Effect of bovine serum albumin on coupling efficiency of mitochondria isolated from the zona glomerulosa of the bovine adrenal cortex in various media was examined polarographically and by EM. Albumin restored the coupling efficiency of mitochondria isolated from the zona glomerulosa regardless of isolation media when succinate or malate was an oxidizable substrate. Respiratory controls greater than 5 were obtained. Albumin had no effect when glutamate, .beta.-hydroxybutylate and pyruvate were the oxidizable substrates. The conditions were found under which mitochondria of the zona glomerulosa stay in the orthodox configuration and yet coupled.