The Physical Chemistry of Silver Staining

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Abstract
It has been shown that silver deposition plays a part in the silver staining process. From this it has been concluded that the rate of reduction of silver within and on histological structures is an important factor. Some factors controlling the rate of reduction, such as the adsorption of silver hydroxide and ammonia, the affinity of silver for proteins, and the protective power of the gel structures have been pointed out. Some simple applications of the ideas to silver staining have been given and two technics described, one making use of piperidine instead of ammonia, the other carrying out the reduction in the presence of the silver solution to facilitate deposition.

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