1H NMR study of the exchangeable protons of the duplex d(GCGTTGCG).d(CGCAACGC) containing a thymine photodimer containing a thymine photodimer
Open Access
- 11 June 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (11) , 4645-4653
- https://doi.org/10.1093/nar/15.11.4645
Abstract
A comparison is presented of the imino proton NMR spectra of the double stranded octamer d(GCGTTGCG).d(CGCAACGC) and the same octamer in which the two central thymine residues occur as a cis-syn thymine dimer. Except for the terminal base pairs all imino protons were detected and assigned in the NMR spectrum. The spectra show that in the thymine dimer duplex, contrary to common belief, all base pairs occur in a hydrogen bonded form, although the hydrogen bonds of the two central AT base pairs are substantially weakened. The melting temperature decreases about 13°C on thymine dimer formation.Keywords
This publication has 6 references indexed in Scilit:
- Hairpin formation in synthetic oligonucleotidesBiochimie, 1985
- Base-base mismatches. Thermodynamics of double helix formation for dCA3XA3G + dCT3YT3G (X, Y = A,C,G,DNucleic Acids Research, 1985
- Molecular Models for DNA Damaged by PhotoreactionScience, 1985
- The structure of d(CGCGAAT[ ]TCGCG)-d(CGCGAATTCGCG): the incorporation of a thymine photodimer into a B-DNA helixNucleic Acids Research, 1984
- Effect of a single thymine photodimer on the oligodeoxythymidylate-polydeoxyadenylate interactionJournal of the American Chemical Society, 1971
- Disappearance of thymine photodimer in ultraviolet irradiated DNA upon treatment with a photoreactivating enzyme from Baker's yeastBiochemical and Biophysical Research Communications, 1962