Subcycling-PCR for Multiplex Long-Distance Amplification of Regions with High and Low GC Content: Application to the Inversion Hotspot in the Factor VIII Gene
Open Access
- 1 December 1998
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 25 (6) , 1022-1028
- https://doi.org/10.2144/98256rr01
Abstract
Previously we described a PCR protocol for detecting the inversion in the factor VIII gene, which is a common cause of Hemophilia A. This PCR assay is challenging due to the size of the amplificati...Keywords
This publication has 0 references indexed in Scilit: