Competitive Inhibition for Amino Acid Uptake by the Indigenous Microflora of Upper Klamath Lake1

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Abstract
The uptake of a specific 14C-amino acid by the heterotrophic microorganisms in the epilimnion of an eutrophic lake was influenced by the presence of other amino acids. The effect of unlabeled serine on 14C-glycine uptake was shown to be caused by competitive inhibition, which changed the interpretation of the kinetic parameters, the turnover time, Tt, and the sum of a transport constant, (Kt + (Sn), and the natural substrate concentration. The maximum velocity of uptake, Vmax, is unaffected by the competitive inhibition.

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