An effect of chloride on (Na + K) co-transport in human red blood cells

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Abstract
In certain conditions internal K can stimulate active Na:K transport and Na:Na exchange through the ouabain-sensitive Na, K exchange pump. The removal of K0 (the main requirement for Na:Na exchange) reduced the ouabain-insensitive Na efflux. In 8 experiments done variously with outdated cells or high and low [K]i, PCMBS[p-chloromercuribenzene sulfonate]-treated cells, the K0-dependent Na efflux ranged from 0.18-0.70 .mu.eq/ml cells per h. This conflicts with the view that the K0-dependent and ouabain-sensitive components of Na efflux are identical. The K0-dependent, ouabain-insensitive Na efflux proceeds through the (Na + K) cotransport system, which shows a dependence on chloride ions.