Further properties and possible mechanism of action of adenosine 5′-triphosphate–d-glucose 6-phosphotransferase from rat liver

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Abstract
Magnesium ions are the most effective bivalent ions in the glucokinase reaction. The molecular weight of rat hepatic glucokinase is 48,000-49,000 as assessed by gel filtration on Sephadex G-100. Anomalous kinetic behavior at low glucose concentrations appears to be due to the formation during the purification procedure of fragments possessing modified catalytic properties, but is unlikely to be of physiological significance. Extension of previous studies suggests that glucokinase catalyzes a reaction of the random Bi Bi type similar to that of yeast hexokinase. The inhibitory effects of various thiol reagents suggest that a thiol group may be involved at or near the binding site of the acceptor molecule.