Monoclonal BALB/c anti‐progesterone antibodies use family IX variable region heavy chain genes
- 1 January 1987
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 17 (1) , 9-13
- https://doi.org/10.1002/eji.1830170103
Abstract
Variable region nucleotide sequences and respective translated amino acid sequences for three heavy chains (DB3, 11/32 and 10/8) and two light chains (DB3 and 11/32) of monoclonal mouse IgG1 anti‐progesterone antibodies have been determined by primer extension mRNA sequencing. The three VH regions exhibit the same rarely observed VH IX gene family and have > 88% homology between them. Two associated light chain sequences are 95% homologous and belong to the VxI group. The N‐terminal twenty two amino acids of the x light chain of the third antibody 10/8 have been determined by automated protein sequencing and are identical to those of 11/32. Thus, these three monoclonal anti‐progesterones derived from separate fusions all use VHIX‐Vx1 gene combinations.Keywords
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