In situ nick translation of human metaphase chromosomes with the restriction enzymes Mspl and Hpall reveals an R-band pattern
- 1 January 1990
- journal article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 54 (3-4) , 132-136
- https://doi.org/10.1159/000132976
Abstract
Several restriction enzymes (HindIll, HaeIII, Mspl, HpaII, EcoRl, Kpnl, and NotI were evaluated for their ability to induce bands in human metaphase chromosomes during in situ nick translation. Mspl and Hpallwere able to induce a completely developed R-band pattern. Preferential cleavage of R-band chromatin is due to the presence of unmethylated CpG-residues present in CpG-rich islands, which are apparently unevenly distributed and mainly concentrated in R-bands.Keywords
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