Establishment of an Estrogen Responsive Rat Pituitary Cell Sub-Line MtT/E-2.

1 January 1999

journal article
Published by Japan Endocrine Society in Endocrine Journal

Vol. 46 (3) , 389-396
https://doi.org/10.1507/endocrj.46.389

Abstract

A rat pituitary tumor sub-line MtT/E-2 was established from a rat pituitary tumor cell line MtT/E. Its growth was found to depend on the presence of estradiol (E₂) in culture media at 10^-13-10^-9M, whereas the original cell line MtT/E proliferated autonomously. The recently discovered PTTG (pituitary tumor transforming gene) is highly expressed in this cell line, although not regulated by E₂. On the other hand, E₂ induced c-myc and cyclin D1 proteins in MtT/E-2, which contains a lot (220±18fmol/mg protein) of estrogen receptor demonstrated by RT-PCR analysis to be predominantly α type. MtT/E-2 secretes growth hormone which is, interestingly, regulated by retinoic acid and dexamethasone rather than thyroid hormones.

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