Detection of Tobacco Mosaic and Tobacco Ringspot Viruses in White Ash Trees by Enzyme-Linked Immunosorbent Assay

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Abstract
Tobacco mosaic virus (TMV) or tobacco ringspot virus (TbRSV), or both, were detected by enzyme-linked immunosorbent assay (ELISA) at various times during the 1981 growing season in tissue samples taken from 21 of 23 white ash trees. Virus infection was not correlated with twig and branch dieback; however, all but 2 trees with viruslike foliar symptoms were infected with either or both viruses. Neither virus was associated consistently with any specific foliar symptom. TbRSV was detected most consistently in root tissue samples. TMV was detected with equal frequency in both root and aboveground tissues. Peak periods of virus detection occurred at 3- to 5-wk intervals throughout the growing season.

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