SYSTEMATIC SEROLOGY AMONG CERTAIN INSECT SPECIES

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Abstract
12 spp. representing 5 families of Orthoptera are compared serologically. Saline extracts of whole ground insects are used as antigens. The antisera are produced in rabbits. Antigen-antibody reactions are conducted using the a technique, i.e., a constant amount of antibody reacted with an antigen dilution series. Comparisons of interfacial test titers and turbidimetric measurement of antigen-antibody reaction over the whole dilution range are given. The Libby Photronreflectometer is used to make the whole curve analysis. The serological results are in accord with taxonomic-paleologic data. In addition, mean percentage relationship values for tests between spp. of the same genus are found to be 54%; for intergeneric tests within the same family, 30%; and for interfamily tests within the same order, 11%. Reciprocal tests show sufficient constancy to justify the use of tissue extracts of insects in serological problems of relationship.
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