Retention of replication fidelity by a DNA polymerase functioning in a distantly related environment
- 22 July 1997
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 94 (15) , 8042-8046
- https://doi.org/10.1073/pnas.94.15.8042
Abstract
The primary structures of the replicative DNA polymerases (gp43s) of bacteriophage T4 and its distant phylogenetic relative RB69 are diverged, retaining only 61% identity and 74% similarity. Nevertheless, RB69 gp43 substitutes effectively for T4 gp43 in T4 DNA replication in vivo . We show here that RB69 gp43 replicates T4 genomes in vivo with a fidelity similar to that achieved by T4 gp43. Furthermore, replication by RB69 gp43 in the distantly related environment does not enhance the mutator activities of mutations in T4 genes that encode other components of the multienzyme DNA replicase. We also show that the fidelities of RB69 gp43 and T4 gp43 are both high in vitro and that they are similarly and sharply reduced in vivo by mutations that eliminate the 3′-exonucleolytic proofreading function. We conclude that gp43 interactions with the other replication proteins are probably nonessential for polymerase fidelity.Keywords
This publication has 54 references indexed in Scilit:
- Crystal Structure of a pol α Family Replication DNA Polymerase from Bacteriophage RB69Cell, 1997
- Proliferating Cell Nuclear Antigen Promotes Misincorporation Catalyzed by Calf Thymus DNA Polymerase δPublished by Elsevier ,1996
- Functional Consequences and Exonuclease Kinetic Parameters of Point Mutations in Bacteriophage T4 DNA PolymeraseBiochemistry, 1996
- Dynamics of Bacteriophage T4 DNA Polymerase Function: Identification of Amino Acid Residues that Affect Switching between Polymerase and 3′ → 5′ Exonuclease ActivitiesJournal of Molecular Biology, 1995
- Modular Organization of T4 DNA PolymerasePublished by Elsevier ,1995
- CONFORMATIONAL COUPLING IN DNA POLYMERASE FIDELITYAnnual Review of Biochemistry, 1993
- General Antimutators are ImprobableJournal of Molecular Biology, 1993
- HeLa cell single-stranded DNA-binding protein increases the accuracy of DNA synthesis by DNA polymerase α in vitroMutation Research/DNA Repair, 1992
- Studies on the biochemical basis of spontaneous mutationJournal of Molecular Biology, 1977
- Spontaneous Mutation: Genetic Control of Mutation Rates in Bacteriophage T4Nature, 1969