Studies on antitumor polysaccharides of Flammulina velutipes (CURT. ex Fr.) sing.II. The structure of EA3 and further purification of EA5.
- 1 January 1982
- journal article
- research article
- Published by Pharmaceutical Society of Japan in Journal of Pharmacobio-Dynamics
- Vol. 5 (8) , 576-581
- https://doi.org/10.1248/bpb1978.5.576
Abstract
An antitumor polysaccharide, EA3 isolated from a Japanese edible mushroom, F. velutipes (Curt ex Fr.) Sing. is composed of D-glucose. Studies to determine its structure were performed by means of partial acid hydrolysis, acetolysis and the chemical analysis of the complete hydrolysates of the fully methylated polysaccharide. Thus, the chemical structure of EA3 was of a .beta.-(1 .fwdarw. 3)-glucan. Another antitumor polysaccharide (EA5) also isolated from F. velutipes was fractionated on a concanavalin A-Sepharose 4B column. The active antitumor component was further purified by column chromatography on Sephadex G-200. Among the polysaccharides isolated the highest molecular weight polysaccharide (EA501) showed the highest rate of antitumor activity. The component sugar of EA501 was D-glucose 42.3%, D-galactose 17.3%, D-mannose 12.2%, D-xylose 6.7% and L-arabinose 14.7%.This publication has 0 references indexed in Scilit: