Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.

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Abstract
The complete nucleotide sequence of the actin gene from S. cerevisiae was determined. The coding region is interrupted by a 304-base-pair intervening sequence that is located within the triplet coding for amino acid 4. DNA sequences of the intron-exon junctions are similar to those found in higher eukaryotes and can be aligned such that the intron starts with the dinucleotide 5''-G-T-3'' and ends with 5''-A-G-3''. Regions of homology within the sequences upstream from the initiation codon and those following the termination codon were detected between the yeast iso-1-cytochrome c gene and the actin gene. As deduced from the nucleotide sequence, yeast actin has 374 amino acid residues. Its primary structure, especially the NH2-terminal third of the protein, is highly conserved during evolution.