Decrease in functional albumin mRNA during estrogen-induced vitellogenin biosynthesis in avian liver.

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Abstract
Translation of rooster liver RNA in a wheat germ extract yielded albumin as one of the cell-free products. Quantitation of albumin mRNA by the translation assay indicated that functional albumin mRNA represents 10% of total liver mRNA activity in control roosters. After estrogen administration, this level decreased in a continuous fashion until functional albumin mRNA represented 5% of total mRNA activity at 12 days. This decrease in functional albumin mRNA was correlated with several parameters of vitellogenin induction. Functional vitellogenin mRNA increased to a maximum at 4 days after hormone treatment and returned to control levels by 12 days. A similar pattern was seen for the hormone-stimulated increase in total mRNA activity. The decrease in functional albumin mRNA persisted after the vitellogenic response of the liver had been completed. The decrease in hepatic albumin synthesis after hormone treatment was apparently due to an estrogen-mediated decrease in the content of albumin mRNA.