Multiplex PCR-Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes
- 1 May 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (5) , 1887-91
- https://doi.org/10.1128/jcm.44.5.1887-1891.2006
Abstract
We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 “unknowns.” The assay identified all target serotypes, but 11, which cross-react with 1 to 4 nonvaccine serotypes, could be distinguished using serotype-specific antisera.Keywords
This publication has 12 references indexed in Scilit:
- Reverse Line Blot Assay for Direct Identification of Seven Streptococcus agalactiae Major Surface Protein Antigen GenesClinical and Vaccine Immunology, 2006
- Simultaneous Detection of Nine Antibiotic Resistance-Related Genes in Streptococcus agalactiae Using Multiplex PCR and Reverse Line Blot Hybridization AssayAntimicrobial Agents and Chemotherapy, 2006
- Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridizationJournal of Medical Microbiology, 2005
- A molecular-capsular-type prediction system for 90 Streptococcus pneumoniae serotypes using partial cpsA-cpsB sequencing and wzy- or wzx-specific PCRJournal of Medical Microbiology, 2005
- Using cpsA–cpsB sequence polymorphisms and serotype-/group-specific PCR to predict 51 Streptococcus pneumoniae capsular serotypesJournal of Medical Microbiology, 2003
- Rapid Screening for Freshwater Bacterial Groups by Using Reverse Line Blot HybridizationApplied and Environmental Microbiology, 2003
- GP5+/6+ PCR followed by Reverse Line Blot Analysis Enables Rapid and High-Throughput Identification of Human Papillomavirus GenotypesJournal of Clinical Microbiology, 2002
- Serotype Identification of Group B Streptococci by PCR and SequencingJournal of Clinical Microbiology, 2002
- Typing of Streptococcus pneumoniae: past, present, and futureThe American Journal of Medicine, 1999
- Diagnosis of Bacteremic Pneumococcal Pneumonia by Amplification of Pneumolysin Gene Fragment in SerumThe Journal of Infectious Diseases, 1995