Interplay between Efflux Pumps May Provide Either Additive or Multiplicative Effects on Drug Resistance
- 1 June 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (11) , 3142-3150
- https://doi.org/10.1128/jb.182.11.3142-3150.2000
Abstract
The effects of simultaneous expression of several efflux pumps on antibiotic resistance were investigated in Escherichia coli and Pseudomonas aeruginosa . Several combinations of efflux pumps have been studied: (i) simultaneous expression of a single-component efflux pump, which exports antibiotics into the periplasm, in combination with a multicomponent efflux pump that accomplishes efflux directly into the external medium; (ii) simultaneous expression of two single-component pumps; and (iii) simultaneous expression of two multicomponent pumps. It was found that when efflux pumps of different structural types were combined in the same cell (the first case), the observed antibiotic resistance was much higher than that conferred by each of the pumps expressed singly. Simultaneous expression of pairs of single-component or multicomponent efflux pumps (the second and third cases) did not produce strong increases in antibiotic resistance.Keywords
This publication has 46 references indexed in Scilit:
- Inhibitors of Efflux Pumps in Pseudomonas aeruginosa Potentiate the Activity of the Fluoroquinolone Antibacterial LevofloxacinJournal of Medicinal Chemistry, 1999
- AcrA is a highly asymmetric protein capable of spanning the periplasm 1 1Edited by I. B. HollandJournal of Molecular Biology, 1999
- The Complete Genome Sequence of Escherichia coli K-12Science, 1997
- The Role ofmex-Gene Products in Antibiotic Extrusion inPseudomonas aeruginosaBiochemical and Biophysical Research Communications, 1997
- Characterization of MexE–MexF–OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosaMolecular Microbiology, 1997
- Overexpression of the mexC–mexD–oprJ efflux operon in nfxB‐type multidrug‐resistant strains of Pseudomonas aeruginosaMolecular Microbiology, 1996
- Expression of Genes Associated with Antibiotic Extrusion in Pseudomonas aeruginosaBiochemical and Biophysical Research Communications, 1995
- Isolation of OprM-deficient mutants ofPseudomonas aeruginosaby transposon insertion mutagenesis: Evidence of involvement in multiple antibiotic resistanceFEMS Microbiology Letters, 1994
- Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdineMolecular Microbiology, 1993
- A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1983