A site-specific single strand endonuclease activity induced by NYs-1 virus infection of aChlorella-like green alga
Open Access
- 25 October 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (20) , 9477-9487
- https://doi.org/10.1093/nar/16.20.9477
Abstract
A site-specific endonuclease was isolated from a eukaryotic Chlorella -like green alga infected with the dsDNA-containing virus NYs-1. The enzyme recognizes the sequence 5'-CC-3' and cleaves 5' to the first C. It cleaves 5'-C m C-3' sequences but not 5'- m CC-3' sequences. The enzyme creates breaks in dsDNA whenever two 5'-CC-3' sequences on opposite strands are close enough for the two strands to separate; when the 5'-CC-3' sequences on opposite strands are further apart only a portion of the strands separate. Consequently, NYs-1 endonuclease does not produce a completely stable DNA digestion pattern. The enzyme probably does not cleave ssDNA and definitely does not cleave ssRNA or dsRNA.Keywords
This publication has 17 references indexed in Scilit:
- 5-Methylcytosine replacing cytosine in the deoxyribonucleic acid of a bacteriophage for Xanthomonas oryzaePublished by Elsevier ,2004
- Characterization of viruses infecting a eukaryotic Chlorella-like green algaVirology, 1986
- Dideoxy DNA sequencing with end-labeled oligonucleotide primersAnalytical Biochemistry, 1984
- Insertion mutations in the dam gene of Escherichia coli K-12Molecular Genetics and Genomics, 1983
- [39] Separation and isolation of DNA fragments using linear polyacrylamide gradient gel electrophoresisPublished by Elsevier ,1980
- [2] Assaying of organisms for the presence of restriction endonucleasesPublished by Elsevier ,1980
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- A site-specific single-strand endonuclease from the eukaryote Chlamydomonas.Proceedings of the National Academy of Sciences, 1977
- Bacteriophage T5-induced endonucleases that introduce site-specific single-chain interruptions in duplex DNA.Proceedings of the National Academy of Sciences, 1976
- Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis*Biochemistry, 1967