Quantification of Niacin and Niacinamide in Vitamin Preparations by Densitometric Thin Layer Chromatography
- 1 December 1986
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 9 (16) , 3423-3431
- https://doi.org/10.1080/01483918608077792
Abstract
A densitometric TLC method was developed for the determination of the B-3 vitamins niacin and niacinamide in commercial vitamin preparations. The method involves removal of excipients by ethanol precipitation, separation of the vitamins by high performance TLC on phosphor-containing silica gel layers, and in situ scanning of sample and standard zones. The per cent recoveries for niacinamide in vitamin products were 96 to 104% and for niacinamide added to products 99 and 102%. Recoveries of added niacin were 99 to 102%, but recoveries of niacin in products ranged from 0 to 74% of the label values. Additional ingredients did not interfere with the determination.This publication has 1 reference indexed in Scilit:
- Thin Layer Chromatographic Identification and Quantitation of Niacin and Niacinamide in Pharmaceutical PreparationsJournal of AOAC INTERNATIONAL, 1985