Analysis of Bulk Tank Milk to Determine the Bacterial Flora of the Mammary Gland of Lactating Cows in a Dairy Herd

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Abstract
Milk samples (5-10 ml) were collected from bulk tanks from 5 dairy herds on 5 consecutive days. Samples were stored frozen and composite samples prepared for each herd for measurement of total bacterial counts. Streptococcus agalactiae, streptococci other than S. agalactiae, pathogenic staphylococci, non-pathogenic staphylococci and coliforms using blood agar, TKT-ferric citrate, tellurite glycine and MacConkey media. Plates were incubated at 37 deg C for 18-24 h, and counts made on plates with 30-300 colonies present. Details of analyses of milk from the 5 herds are given and the results discussed in terms of milk and detection of major pathogens present. The method is recommended for herd evaluation and as an indication of further tests that need to be made.

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