High-performance liquid chromatographic determination of bromazepam in human plasma
- 1 January 1989
- journal article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 114 (5) , 639-641
- https://doi.org/10.1039/an9891400639
Abstract
An assay using high-performance liquid chromatography has been developed for the determination of bromazepam in plasma. After a single-step extraction from basified samples with dichloromethane, using decarboxyloflazepate as an internal standard, samples were analysed using a reversed-phase Nova Pak 5-µm column with a mobile phase of methanol-phosphate buffer (60 + 40) adjusted to pH 7.6. The drugs were detected at 239 nm and the limit of detection was found to be 3 µg l–1 for bromazepam. The method is simple, rapid and sensitive and permits bromazepam levels in clinical and pharmacokinetic studies to be monitored.Keywords
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