PITFALLS IN IMMUNOFLUORESCENCE TESTING IN CANINE DERMATOLOGY

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 73  (2) , 131-136
Abstract
Indirect immunofluorescence testing for pemphigus-like antibodies was performed on 50 dogs with various nonpemphigus dermatologic diseases, 25 dogs with various nondermatologic diseases, and 25 normal dogs. One dog (generalized demodicosis) was positive for pemphigus-like antibodies at a titer of 1:10. Canine pemphigus-like antibodies are a potential source of misinterpretation and misdiagnosis in indirect immunofluorescence testing. Direct immunofluorescence testing for IgG, IgA, IgM and C3 [complement component 3] was performed on the footpads of 11 normal dogs. Granular deposition of IgM at the basement membrane zone was demonstrated in 5 of the 11 dogs. Direct immunofluorescence testing of canine footpads using only polyvalent Ig antisera or anti-IgM antisera may lead to misinterpretation and misdiagnosis in up to 45% of all dogs tested.

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