3'-DEOXY-3'-FLUOROTHYMIDINETRIPHOSPHATE - INHIBITOR AND TERMINATOR OF DNA-SYNTHESIS CATALYZED BY DNA POLYMERASE-BETA, TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE AND DNA-POLYMERASE I
- 1 January 1985
- journal article
- research article
- Vol. 44 (10) , K63-K73
Abstract
3''-deoxy-3''-fluorothymidine-5''-triphosphate (dFTTP) was examined with respect to its capacity to inhibit and to terminate DNA-synthesis catalysed by DNA polymerases .alpha. and .beta., terminal deoxynucleotidyl transferase (TdT) and Escherichia coli DNA polymerase I (Klenow fragment). In analogy with 2'', 3''-dideoxythymidine-5''-triphosphate (ddTTP) it was found that DNA polymerase .beta. was much more sensitive to dFTTP inhibition than DNA polymerase .alpha.. Moreover, the activity of TdT is strongly inhibited by both dFTTP and ddTTP, whereas the two compounds displayed a lower inhibitory potency on DNA polymerase I. Except for DNA polymerase .alpha., the other investigated DNA polymerases are able to incorporate dFTTP into poly (dA) .cntdot. oligo (dT) and oligo (dG), respectively. The specific chain-terminating activity of dFTTP has proved for DNA sequencing to be at least equivalent to ddTTP.This publication has 12 references indexed in Scilit:
- Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.Proceedings of the National Academy of Sciences, 1982
- Terminal deoxynucleotidyltransferase of 60,000 daltons from mouse, rat, and calf thymus. Purification by immunoadsorbent chromatography and comparison of peptide structures.Journal of Biological Chemistry, 1981
- Inhibition of deoxyribonucleic acid polymerases of human leukemic leukocytes by 2',3'-dideoxythymidine triphosphateBiochemical Pharmacology, 1980
- Involvement of eucaryotic deoxyribonucleic acid polymerases .alpha. and .gamma. in the replication of cellular and viral deoxyribonucleic acidBiochemistry, 1979
- Involvement of DNA polymerase alpha in simian virus 40 DNA replication.Journal of Biological Chemistry, 1978
- Effects of 3′-deoxy-3′-fluorothymidine on proliferation kinetics of Ehrlich ascites tumour cells in vitro (comparison with other cytostatic drugs)Published by Elsevier ,1978
- PURIFICATION OF RNA-DIRECTED DNA-POLYMERASE FROM MOUSE SPLEEN INFECTED WITH RAUSCHER LEUKEMIA-VIRUS1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .9. POLYMERASE FORMED AFTER T2 BACTERIOPHAGE INFECTION OF ESCHERICHIA COLI - NEW ENZYME1962
- ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .8. FREQUENCIES OF NEAREST NEIGHBOR BASE SEQUENCES IN DEOXYRIBONUCLEIC ACID1961