Low-angle neutron scattering from chromatin subunit particles
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (11) , 2163-2176
- https://doi.org/10.1093/nar/2.11.2163
Abstract
Monomer chromatin particles containing 140 base pairs of DNA and eight histone molecules have been studied by neutron scattering. From measurements in various H2O/D2O mixtures, radii of gyration and the average scattering density of the particle were determined. The radius of gyration under conditions when scattering from the DNA dominates is 50A, and when scattering from the protein dominates, 30A. Consequently the core of the particle is largely occupied by the histones while the outer shell consists of DNA together with some of the histone.Keywords
This publication has 18 references indexed in Scilit:
- Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonucleasePublished by Elsevier ,2004
- Comparison of the digestion of nuclei and chromatin by staphylococcal nucleaseBiochemistry, 1975
- Comparison of neutron and X-ray scattering of dilute myoglobin solutionsJournal of Molecular Biology, 1975
- Electron microscopic and biochemical evidence that chromatin structure is a repeating unitCell, 1975
- The subunit structure of the eukaryotic chromosomeNature, 1975
- A neutron scattering study of the distribution of protein and RNA in the 30 S ribosomal subunit of Escherichia coliJournal of Molecular Biology, 1975
- The effect of deuterium ion concentration on the properties of sarcoplasmic reticulumThe Journal of Membrane Biology, 1974
- Chromatographic separation of chromatin subunitsBiochemical and Biophysical Research Communications, 1974
- Electron microscopy of chromatin subunit particlesBiochemical and Biophysical Research Communications, 1974
- Chromatin Structure: A Repeating Unit of Histones and DNAScience, 1974