ELECTRON MICROSCOPIC HISTOCHEMISTRY OF CHOLINESTERASE ACTIVITY OF VATER-PACINI CORPUSCLE

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Abstract
The cholinesterase activity of the Vater-Pacini corpuscle in the periosteum of mouse fibula was histochemically studied by light microscopy and EM. The enzyme activity was confined to the inner core of the corpuscle. The reaction product was found in the perineural spaces between the nerve ending and apposing inner core lamellae, and in the interlamellar spaces. The reaction product was found in the nuclear envelope and in the cisterns of the rough endoplasmic reticulum of the inner core cells. The enzyme activity was totally suppressed by eserine 10-4 M and isoOMPA [tetraisopropyl pyrophosphoramide] 10-4 M. No enzyme activity was demonstrated in the outer core and capsule. The nerve ending showed no distinct enzyme activity. The mouse Pacini corpuscle apparently has non-specific cholinesterase activity in its inner core, and the enzyme may be synthesized in the inner core cells and released into the perineural and interlamellar spaces.