Micromethod for chromosome preparations from Chinese hamster lymphocytes
- 1 January 1979
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 23 (3) , 194-200
- https://doi.org/10.1159/000131325
Abstract
Chromosome preparations from Chinese hamster lymphocytes were made by culturing for 2 to 6 days 50,000 to 800,000 lymphocytes in flat-bottomed Cooke microtiter plates in 0.1 or 0.2 ml Roswell Park Memorial Institute-1640 medium (RPMI-1640) supplemented with 5–40 µl PHA/ml, 20% fetal calf serum (FCS), and 40 µM 2-mercapto-ethanol. Depending on blood volume and cell concentrations used, it was possible to obtain up to 1400 well-spread metaphases from one venepuncture.Keywords
This publication has 0 references indexed in Scilit: