Cloning and partial nucleotide sequence of human immunoglobulin mu chain cDNA from B cells and mouse-human hybridomas.
- 1 October 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (10) , 6027-6031
- https://doi.org/10.1073/pnas.77.10.6027
Abstract
Purified mRNA coding for .mu. and .kappa. human immunoglobulin polypeptides were translated in vitro and their products were characterized. The .mu.-specific mRNA, derived from both human lymphoblastoid cells (GM607) and from a mouse-human somatic cell hybrid secreting human .mu. chains (.alpha.D5-DH11-BC11), were copied into c[complementary]DNA and inserted into the plasmid pBR322. Several recombinant cDNA that were obtained were identified by a combination of colony hybridization with labeled probes, in vitro translation of plasmid-selected .mu. mRNA, and DNA nucleotide sequence determination. One recombinant DNA, for which the sequence was partially determined, contains the codons for part of the C3 constant region domain through the carboxy-terminal piece (155 amino acids total) and the entire 3'' noncoding sequence up to the poly(A) site of the human .mu. mRNA. The sequence A-A-U-A-A occurs 12 nucleotides prior to the poly(A) addition site in the human .mu. mRNA. Considerable sequence homology is observed in the mouse and human .mu. mRNA 3'' coding and noncoding sequences.This publication has 35 references indexed in Scilit:
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