Whole-cell recordings of chloride currents in cultured human skeletal muscle

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Abstract
Chloride currents in human myoballs were investigated with the tight-seal whole-cell recording method in a wide range of membrane voltages (−125 to +145 mV). Two current components having different kinetics could be distinguished. In more than 90% of the myoballs the following results were obtained. At negative potentials, the amplitude of the Cl− current was small and independent of time. The amplitude of the current increased as the membrane potential was made more positive. At potentials positive to +75mV, the current increased monoexponentially with time. Inactivation occurred only during very long (>3 s) pulses. When such a test pulse was preceded by a conditioning pulse to +60 mV, the current at potentials more than +90 mV was markedly smaller than in the absence of a prepulse, and no activation was provoked by strongly pulses. Recovery from inactivation could only be measured at potentials negative to −40mV. The Cl− conductance at −85mV was 5.9±3.64 μS/cm2 (SD; n=10). In about 5% of the myoballs a kinetically different current was visible, characterized by fast inactivation at highly positive potentials. The current amplitudes were substantially larger in such cases, the Cl− conductance at −85mV being 12.2±9.02 μS/cm2 (n=4).