Examination and Photography of Donor Corneal Endothelium

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Abstract
In a method for the routine examination of the endothelium of donor corneas before transplantation, the donor endothelial cells are examined and photographed as they undergo preservation or culture in liquid media. A specular microscope with a modified objective lens is used (working distance, approximately 9 mm). The donor corneas are kept in a special storage container that allows a direct view of the endothelium from outside the container. The endothelium of any cornea preserved in a liquid medium may thus be examined and photographed at high magnification without opening the storage container.

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