Microanalysis of plant mitochondrial protein synthesis products:
- 1 January 1984
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 3 (6) , 445-452
- https://doi.org/10.1007/bf00033392
Abstract
A mitochondrial fraction obtained from 0.5 g of leaves was purified on a 0.75 ml Percoll gradient and used for an in vitro mitochondrial protein synthesis assay in the presence of [35S] methionine. A set of 15 to 20 labeled polypeptides were revealed by autoradiography after sodium dodecylsulfate polyacrylamide gel electrophoresis. This could be applied at an early growth stage by using a few leaves from individual seedlings. It revealed the presence of variant mitochondrially translated polypeptides in green leaves of cytoplasmic male sterile lines from various cultivated plants of large economic importance: maize, wheat, sugar beet, tobacco and faba bean. This non-destructive microanalysis is thus of general use and opens new possibilities for rapid and large mass screening of mitochondrial parameters such as male sterility.This publication has 22 references indexed in Scilit:
- Ertyhromycin as a tool for discriminating in vivo between mitochondrial and chloroplastic protein synthesis in Nicotiana sylvestrisPlant Science Letters, 1983
- Mitochondrial Modifications Associated with the Cytoplasmic Male Sterility in Faba BeansEuropean Journal of Biochemistry, 1982
- Mitochondrial Genome Organization and Expression in Higher PlantsAnnual Review of Plant Physiology, 1982
- Method to Obtain a Chlorophyll-free Preparation of Intact Mitochondria from Spinach LeavesPlant Physiology, 1980
- Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusionNature, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Separation of Mitochondria from Contaminating Subcellular Structures Utilizing Silica Sol Gradient CentrifugationPlant Physiology, 1979
- In Vitro Study of Mitochondrial Protein Synthesis during Mitochondrial Biogenesis in Excised Plant Storage TissuePlant Physiology, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949