INDUCTION OF TOLERANCE IN ADULT GUINEA-PIGS BY 2 ANTIGEN DOSAGE LEVELS AND BY ANTIGENIC-COMPETITION

  • 1 January 1975
    • journal article
    • research article
    • Vol. C126  (5-6) , 553-563
Abstract
The effect of prior injection of increasing doses of dinitrophenylated bovine serum albumin (DNP-BSA) mixed in Freund''s complete (FCA) or incomplete (IFA) adjuvants on the antibody response to a subsequent challenge with an optimal immunizing dose of DNP-BSA in FCA was examined and compared with the effect of competition with the unrelated antigen bovine .gamma.-globulin (BGG). An unresponsive state was induced by pretreatments with a minimal dose of antigen, a large antigen excess or a competitive mixture in FCA. The specificity of this unresponsiveness was ascertained by the normal response of pretreated animals to the unrelated antigen arsanilic-human albumin (ARS-HSA). Prior injection of a minimal dose (0.2 .mu.g), an antigen excess (20 mg) of DNP-BSA or an optimal dose (20 .mu.g) of DNP-BSA mixed with an excess of BGG, incorporated in IFA, also induced a specific tolerance to DNP-BSA. The former treatment induced a low zone tolerance; both latter treatments induced a high zone tolerance.

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