Analysis of Cell Surface Carbohydrate Expression Patterns in Normal and Tumorigenic Human Breast Cell Lines Using Lectin Arrays
- 20 June 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 79 (15) , 5698-5702
- https://doi.org/10.1021/ac070423k
Abstract
Cell surface carbohydrates play important roles in a wide variety of biological processes including cell adhesion, fertilization, differentiation, development, and tumor cell metastasis. Lectins are proteins of nonimmune origin which recognize and bind to specific carbohydrate structural epitopes. We have recently described the development and use of lectin arrays as tools for the elucidation of the carbohydrate structures expressed on cell surfaces. In the present work this technology is employed for the characterization of differences in carbohydrate expression patterns on normal and tumorigenic human breast cell lines, as well as on sublines differing in their tendency to “home” to different tissues during metastasis. Significant differences were observed, including changes that correlate with metastatic potential as well as with tissue-specific homing of metastatic cells.Keywords
This publication has 48 references indexed in Scilit:
- Specific Capture of Mammalian Cells by Cell Surface Receptor Binding to Ligand Immobilized on Gold Thin FilmsJournal of Proteome Research, 2006
- Altered mRNA Expression of Sialyltransferase in Squamous Cell Carcinomas of the CervixGynecologic Oncology, 2001
- Chemical GlycobiologyScience, 2001
- β-galactoside α2,6 sialyltransferase in human colon cancer: contribution of multiple transcripts to regulation of enzyme activity and reactivity withsambucus nigra agglutininInternational Journal of Cancer, 2000
- Altered carbohydrate composition in colorectal adenomas and carcinomas: Histochemical characterization of N-acetylgalactosamine,l-fucose, and o-acetylated sialic acidThe Esophagus, 1996
- Probe-Immobilized Affinity Chromatography/Mass SpectrometryAnalytical Chemistry, 1995
- Quantitative lectinhistochemical and immunohistochemical studies on the occurrence of alpha(2,3)- and alpha(2,6)-linked sialic acid residues in colorectal carcinomas. Relation to clinicopathologic featuresCancer, 1995
- Expression of PNA‐binding sites on specific glycoproteins by human melanoma cells is associated with a high metastatic potentialJournal of Cellular Biochemistry, 1994
- A murine model for evaluating metastatic potential: Characterization of a 90–110-kDa metastasis-binding proteinExperimental Gerontology, 1992
- Lectin Binding in Human Breast Cancer: Clinical and Pathologic Correlations with Fluorescein-Conjugated Peanut, Wheat Germ and Concanavalin A BindingOncology, 1988