Effect of Intravenous Administration of D‐Lysergic Acid Diethylamide on Initiation of Protein Synthesis in a Cell‐Free System Derived from Brain

Publisher Website
Google Scholar
Abstract
An initiating cell-free protein synthesis system derived from brain was utilized to demonstrate that the i.v. injection of LSD to rabbits resulted in a lesion at the initiation stage of brain protein synthesis. Three inhibitors of initiation, edeine, poly(I) and aurintricarboxylic acid were used to demonstrate a reduction in initiation-dependent amino acid incorporation in the brain cell-free system. One hour after LSD injection, there was also a measurable decrease in the formation of 40S and 80S initiation complexes in vitro, using either [35S]methionine or [35S]Met-tRNAf. Analysis of methionine pool size after LSD administration indicated there was no change in methionine levels. Analysis of the formation of initiation complexes in the brain cell-free protein synthesis system prepared 6 h after LSD administration indicated that there was a return to control levels at this time. The effects of LSD on steps in the initiation process are thus reversible.