THE LASER AS A TOOL FOR ISOLATING SINGLE CELLS

Abstract
A laser pulse of 15 millijoules was used to rupture the wall of the target cell of the protonema of the moss Bryum stenotrichum C.M. and cause the protoplast to be ejected. A technique for isolating cells from an organized system, such as a moss protonema, by the laser beam is described. The behavior of the isolated apical and subapical cells in culture has been compared.

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