Secretion of photosynthetic products by carrot tissue cultures

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Abstract
Green carrot callus cultures when exposed to 14CO2 in a liquid medium showed ligh-dependent 14C-incorporation into sucrose, glutamine and malic acid. About 5% of total 14C fixed in a 3 h period appeared in these products in the bathing medium; this was not due to tissue damage. Kinetic studies showed that the release occurred from a metabolic and not a storage compartment. The effects of DCMU, temperature and fluoroacetate demonstrated that release from this compartment was under respiratory and not photosynthetic control.