Determination of the amount of small messenger ribonucleic acid-containing particles free in liver cytoplasm
- 30 September 1975
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 152 (1) , 51-56
- https://doi.org/10.1042/bj1520051
Abstract
To assess the contribution made by mRNA-containing particles to the heterogeneity previously observed among rat liver 40S ribonucleoprotein particles, the amount of poly(A)-containing RNA in subribosomal particles was determined. RNA was labeled with orotate in vivo for 24 h and then for 50 min. Poly(A)-containing RNA was trapped on filters impregnated with poly(U). Verly little poly(A)-containing RNA was found in conventionally prepared ribonucleoprotein particles after fractionation in sucrose. After preparation of ribonucleoprotein particles by sedimentation through 1 M-sucrose in the presence of 0.15 M-KCl or by precipitation with Mg2+ as described by Leitin and Lerman, amounts of poly(A)-containing RNA were similar to amounts of mRNA found by other workers in total ribonucleoprotein particles. Even in such preparations, less than 5% of the total rapidly labeled RNA in native subribosomal-particle fractions was mRNA. mRNA-containing particles apparently make up only a very small part of the population of subribosomal particles in liver.This publication has 21 references indexed in Scilit:
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