Characterization of Monoclonal Antibodies against Human Parvovirus B19
- 1 July 1989
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 33 (7) , 561-567
- https://doi.org/10.1111/j.1348-0421.1989.tb02006.x
Abstract
Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.This publication has 9 references indexed in Scilit:
- Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liverJournal of Virology, 1989
- Translational regulation of B19 parvovirus capsid protein production by multiple upstream AUG triplets.Journal of Biological Chemistry, 1988
- Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selectionJournal of Virology, 1988
- Structure and Mapping of the DNA of Human Parvovirus B19Journal of General Virology, 1987
- Novel transcription map for the B19 (human) pathogenic parvovirusJournal of Virology, 1987
- Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assayJournal of Clinical Microbiology, 1986
- New Monoclonal Antibodies That Define Multiple Epitopes and a Human‐Specific Marker on the Interleukin 2 Receptor Molecules of PrimatesMicrobiology and Immunology, 1986
- Identification of a glycoprotein, gp21, of adult T cell leukemia virus by monoclonal antibody.The Journal of Immunology, 1984
- Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV)Epidemiology and Infection, 1983