Human B cell line deficient in the expression of B cell‐specific glycoproteins (GP 27,35)

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Abstract
A human B lymphoid cell line, P3HR‐1, expresses only low levels of the 27 000 and 35 000 mol.wt. B cell‐specific glycoproteins (GP 27,35). Indirect antibody‐binding and quantitative absorption tests with a xenoantiserum against the antigens showed that P3HR‐1 cells have on their surface about 1 % of the amount found on other human B lymphoblastoid cell lines. The deficit of the glycoproteins on the surface of P3HR‐1 cells could be accounted for by a reduced. rate of synthesis in these cells. A simple relationship between the reduced expression of GP 27,35 on P3HR‐1 cells and their inability to bind Epstein‐Barr virus (EBV) or express complement receptors was excluded because other B lymphoid cells which expressed neither virus‐binding sites nor complement receptor had normal amounts of GP 27,35 on their surface. However, antibodies against GP 27,35 could block the absorption of EBV by EBV receptor‐positive B cells.