Variations in mitochondrial DNA organisation between normal and male-sterile cytoplasms of maize
Open Access
- 10 May 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (9) , 1999-2008
- https://doi.org/10.1093/nar/8.9.1999
Abstract
Mitochondrial DNA from male-sterile lines of maize carrying S cytoplasm contains two small DNA species which are absent from N (fertile), and other male-sterile cytoplasms. Portions of these species have been purified and amplified by constructing recombinant plasmids in vitro . Probes made with these plasmids have been used to demonstrate; i) a homologous region in the N mitochondrial genome, which may indicate the origin of the S specific DNA species, ii) two other DNA species present in low amounts in S cytoplasm only. iii) the absence of strong homology to the S specific DNA species in mitochondria from C and T male-sterile cytoplasms.Keywords
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