Cloning, expression, and localization of a novel γ‐adaptin‐like molecule

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Abstract
We describe the cloning, expression, and localization of γ2‐adaptin, a novel isoform of γ‐adaptin. The predicted human and mouse γ2‐adaptin proteins are ∼90 kDa and 64.4% and 61.7% identical to γ‐adaptin, respectively. γ2‐Adaptin was localized to the Golgi, its localization distinct from γ‐adaptin. The membrane association of γ‐ and γ2‐adaptin could further be distinguished by differential sensitivity to the fungal metabolite brefeldin A, γ2‐adaptin binding being insensitive to drug treatment. Together, these results suggest that γ2‐adaptin plays a role in membrane transport distinct from that played by γ‐adaptin.